Journal: iScience

Article Title: Human induced pluripotent stem cell-derived closed-loop cardiac tissue for drug assessment

doi: 10.1016/j.isci.2024.108992

Figure Lengend Snippet:

Article Snippet: human iPSCs (253G1) were obtained from Riken, Japan and maintained in primate embryonic stem cell medium (ReproCELL, Kanagawa, Japan) with 5 ng/mL human basic fibroblast growth factor (bFGF; ReproCELL) on mouse embryonic fibroblast cells (ReproCELL) treated with mitomycin C (ReproCELL).

Techniques: Recombinant, Modification, Software, Control, Microscopy, Imaging, Flow Cytometry, Transmission Assay

Journal: Theranostics

Article Title: High OGT activity is essential for MYC-driven proliferation of prostate cancer cells

doi: 10.7150/thno.30834

Figure Lengend Snippet: Identification of transcription factors that bind to O-GlcNAc marked chromatin. A) Distribution of differently modified histone 3 reads within ±2kb of O-GlcNAc peak. B) . The top 3 motifs enriched for the O-GlcNAc ChIP-seq data showing similarity with known previously identified transcription factor motifs from published datasets. C) O-GlcNAc ChIP-seq consensus overlap with MYC ChIP-seq data reported in this study. D) O-GlcNAc ChIP-seq consensus overlap with MYC ChIP-exo dataset reported previously (GSE73994). E) OSMI-2 disrupts interaction between MYC and HCF-1. Immunoprecipitation (IP) of MYC. IgG denotes negative control. MYC was overexpressed by addition of doxycycline using the LNCaP-MYC cell line for 4 hours either in the presence or absence of 40µM OSMI-2. Experiment was repeated four times. F) Knockout of OGT disrupts the interaction between MYC and HCF-1. Experiment was performed in a mouse embryonic fibroblast cell line that has been genetically engineered to enable removal of OGT gene by addition of 0.5µM Tamoxifen (Tam). After two days of DMSO- or Tam-treatments, cell lysates were prepared and used for immunoprecipitation. Data shown is representative of two biological replicates. G) Overlap of O-GlcNAc (this study), MYC (this study) and HCF-1 (ENCSR000ECH) ChIP-seq data.

Article Snippet: Tamoxifen-inducible OGT knockout mouse embryonic fibroblast (MEF) cell line was obtained from Dr. Natasha Zachara at the CardioPEG CoreC4 (NHLBI P01 HL107153) at Johns Hopkins University School of Medicine .

Techniques: Modification, ChIP-sequencing, Immunoprecipitation, Negative Control, Knock-Out

Journal: Cell

Article Title: Error-prone replication through UV lesions by DNA polymerase θ protects against skin cancers

doi: 10.1016/j.cell.2019.01.023

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Cell Lines and Cell Culture Normal human fibroblast (GM00637), XPA deficient human fibroblast (GM04429), XPV deficient human fibroblast (GM03617) and mouse embryonic fibroblast cells were grown in DMEM medium (GenDEPOT) containing 10% fetal bovine serum (GenDEPOT) and 1% Antibiotic-Antimyocotic (GenDEPOT).

Techniques: Knock-Out, Staining, Transfection, Construct, Plasmid Preparation, Purification, DNA Ligation, DNA Purification, Proliferation Assay, Single Cell Gel Electrophoresis, Cell Culture, Software